MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1.
Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage KM, Salkeni MA, Ruppert JM
Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62575 | pMIR-Report-RASA1-3'UTR | Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR. |
| 62576 | pMIR-Report-RASA1-3'UTR (mut - miR-206 site) | Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated. |
| 62577 | pMIR-Report-SPRED1-3'UTR (WT - miR-206 site) | Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site |
| 62579 | pMIR-Report-SPRED1-3'UTR (containing miR-21 sites) | Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2) |
| 62580 | pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1) | Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated. |
| 62581 | pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2) | Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated. |