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CoinFLP: a system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila.

Bosch JA, Tran NH, Hariharan IK
Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
52889pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression
52890pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Antibodies from Article