CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi.

Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL
MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
62543	pTrex-b-NLS-hSpCas9	Expression of Cas9 in T. cruzi
62544	pTrex-n-eGFP	Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome