Phosphorylation of the RNase III enzyme Drosha at Serine300 or Serine302 is required for its nuclear localization.
Tang X, Zhang Y, Tucker L, Ramratnam B
Nucleic Acids Res. 2010 Oct;38(19):6610-9. doi: 10.1093/nar/gkq547. Epub 2010 Jun 16.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62520 | GFP-Drosha | wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites |
| 62521 | GFP-Drosha1-390 | C-terminal aa391-1374 of Drosha is deleted |
| 62522 | GFP-Drosha1-850 | C-terminal aa851-1374 of Drosha is deleted |
| 62523 | GFP-Drosha218-1374 | N-terminal aa1-217 of Drosha is deleted |
| 62524 | GFP-Drosha270-1374 | N-terminal aa1-269 of Drosha is deleted |
| 62526 | GFP-DroshaS300A | Serine 300 of Drosha is mutated to alanine |
| 62527 | GFP-DroshaS302A | Serine 302 of Drosha is mutated to alanine |
| 62528 | GFP-DroshaS300E | Serine 300 of Drosha is mutated to glutamic acid |
| 62529 | GFP-DroshaS302D | Serine 302 of Drosha is mutated to aspartic acid |
| 62530 | GFP-DroshaS300/302A | Serines 300 and 302 of Drosha are mutated to alanines |
| 62531 | GFP-DroshaS300E/S302D | Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid |
| 62532 | GFP-Drosha270-1374delNLS2 | N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted |
| 62533 | GFP-Drosha270-1374S300/302A | N-terminal aa1-269 of Drosha is deleted and serines 300 &302 are mutated to alanine |