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Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo.

Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR
Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62372pET-(-30)GFP-9xGGS-Cre-6xHisExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells
62373pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHisExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
62374pET-Cas9-6xHisExpression of Cas9-6xHis in bacterial cells
62933pET-Cas9-NLS-6xHisExpression of Cas9-NLS-6xHis in bacterial cells
62934pET-NLS-Cas9-6xHisExpression of NLS-Cas9-6xHis in bacterial cells
62935pET-dCas9-VP64-6xHisExpression of dCas9-VP64-6xHis in bacterial cells
62936pET-6xHis-(-30)GFPExpression of 6xHis-(-30)GFP in bacterial cells
62937pET-6xHis-(pos36)GFPExpression of 6xHis-(+36)GFP in bacterial cells
62938pET-(pos36)GFP-9xGGS-Cre-6xHisExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells
62939pET-Cre-6xHisExpression of Cre-6xHis in bacterial of cells

Antibodies from Article