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Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling.

Jan CH, Williams CC, Weissman JS
Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521. Epub 2014 Nov 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62358pJW1504RPS2 HA-TEV-AVI pop-in/pop-out plasmid
62359pJW1505RPL16a HA-TEV-AVI pop-in/pop-out plasmid
62360pJW1506RPL16b HA-TEV-AVI pop-in/pop-out plasmid
62361pJW1507pFA6a -mVenus-BirA::HIS5
62362pJW1508pFA6a BirA-mVenus-SSH1::HIS5
62363pJW1509pFA6a BirA-heh2 linker-SSH1::HIS5
62364pJW1510pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5
62365pJW1511pEF1a-puro-T2A-ATHmmuRPL10A
62366pJW1512N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß
62367pJW1234pRS305 pPGK1-BirA::LEU2

Antibodies from Article