Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker.

Behnke MS, Khan A, Sibley LD
Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID	Plasmid	Purpose
59855	pSAG1::CAS9-U6::sg290860-6	Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.