Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells.
Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT, Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. Epub 2014 Nov 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62063 | pGEX6P2-MDM2 (17-125) | Expression of human MDM2 (17-125) in e. coli |
| 62064 | pGEX6P2-MDMX (23-111) | Expression of human MDM4 (residues 23-111) in e. coli |
| 62065 | pGEX6P2-MDMX (1-120) | Expression of human MDM2 (1-120) in e. coli |
| 62066 | pET28A-p53 (1-73) (P12A P13A) | Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli |
| 62067 | pET28A-p53 (1-73) (PPP) | Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli |
| 62068 | pET28A-p53 (1-73) (P27A) | Expression of human p53 (1-73) (P27A) in e. coli |
| 62069 | pET28A-p53 (1-73) (P to A) | Expression of human p53 (1-73) (all P to A) in e. coli |
| 62080 | pET28-p53 (1-90) (72P) | Expression of human p53 (1-90) (72P) in e. coli |
| 62081 | pGEX6P2-p53 (1-90) (72R) | Expression of human p53 (TAD residues 1-90) (72R) in e. coli |