Designed Phosphoprotein Recognition in Escherichia coli.
Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. Epub 2014 Oct 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61965 | pNAS1B split mCherry N-link/link-C | Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein) |
| 61966 | pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C | Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK)) |
| 61967 | pNAS1B split mCherry N-MESVD/TRAP(RK)-C | Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD |
| 61968 | pNAS1B | Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors |