A rapid and robust method for selective isotope labeling of proteins.

Lin MT, Sperling LJ, Frericks Schmidt HL, Tang M, Samoilova RI, Kumasaka T, Iwasaki T, Dikanov SA, Rienstra CM, Gennis RB
Methods. 2011 Dec;55(4):370-8. doi: 10.1016/j.ymeth.2011.08.019. Epub 2011 Sep 8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
61906	ML2	C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.
61907	ML3	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted.
61908	ML6	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE, avtA genes deleted.
61909	ML8	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted.
61910	ML12	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE avtA aspC genes deleted.
61911	ML14	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.
61912	ML17	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted.
61914	ML21	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG tyrA genes deleted.
61915	ML24	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA genes deleted.
61916	ML25	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA asnB genes deleted.
61917	ML26	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH genes deleted.
61918	ML31	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA genes deleted.
61919	ML36	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG metA genes deleted.
61920	ML40K1	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA genes. deleted. Contains pACYC plasmid.
61921	ML42	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnB genes deleted.
61922	ML43	C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB genes deleted.