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Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.

Peng M, Yin N, Li MO
Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61829pInducer21 Flag-Sesn2Dox inducible lentivirial expression of Flag-Sesn2 (mouse)
61865pInducer21 Flag-Sesn1bDox inducible lentivirial expression of Flag-Sesn1b (mouse)
61866pInducer21 Flag-Sesn3Dox inducible lentivirial expression of Flag-Sesn3 (mouse)
61867pcDNA3.1 Flag-Sesn1bTransient overexpression of Flag-Sesn1b (mouse)
61868pcDNA3.1 Flag-Sesn2Transient overexpression of Flag-Sesn2 (mouse)
61869pcDNA3.1 Flag-Sesn3Transient overexpression of Flag-Sesn3 (mouse)
61870pInducer21 Flag-Sesn2 419A/422A/426ADox inducible lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant
61871pLJM1 Flag-Sesn2Lentivirial expression of Flag-Sesn2 (mouse)
61872pLJM1 Flag-Sesn2 419A/422A/426ALentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant
61873pGEX-6p-1 Sesn2bacterial expression of a GST-Sesn2 (mouse)
61874pGEX-6p-1 Sesn1bbacterial expression of a GST-Sesn1b (mouse)
61875pGEX-6p-1 Sesn3bacterial expression of a GST-Sesn3 (mouse)

Antibodies from Article