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In Vivo Proximity Labeling for the Detection of Protein-Protein and Protein-RNA Interactions.

Beck DB, Narendra V, Drury WJ 3rd, Casey R, Jansen PW, Yuan ZF, Garcia BA, Vermeulen M, Bonasio R
J Proteome Res. 2014 Dec 5;13(12):6135-43. doi: 10.1021/pr500196b. Epub 2014 Oct 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61723pINTO-N-SAInducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)
61725pINTO-NSA::mEzh2Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)
61726pINTO-NSA::hSNRNP70Expression of human SNRNP70 fused to monomeric streptavidin (N-terminus)
65924pINTO-N-FHInducible expression of proteins fused to an N-terminal Flag-HA tag (FH)
65925pINTO-NFH::mEzh2Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)
65926pINTO-NFH::hSNRNP70Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

Antibodies from Article