Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning.
Luo Y, Lin L, Bolund L, Sorensen CB
Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. eCollection 2014 May.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
51422 | pGolden-Neo | Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction |
51423 | pGolden-Hyg | Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction |
51424 | pGolden-AAV | Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction |