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Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning.

Luo Y, Lin L, Bolund L, Sorensen CB
Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. eCollection 2014 May. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
51422pGolden-NeoContain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction
51423pGolden-HygContain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction
51424pGolden-AAVContain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

Antibodies from Article