Directed evolution of APEX2 for electron microscopy and proximity labeling.

Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
79057	MICU-APEX2	A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
79056	APEX2-OMM	Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
79055	ERM-APEX2	Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
72558	pTRC-APEX2	Expression of cytosolic APEX2 in bacteria
72480	mito-V5-APEX2	Expresses APEX2 in mitochondria
66172	APEX2 - Actin in pEGFP	APEX2 fused to the N-terminus of human beta actin
66171	APEX2 - Tubulin in pEGFP	APEX2 fused to the N-terminus of alpha tubulin
66170	Vimentin - APEX2 in pECFP	APEX2 fused to the C-terminus human vimentin
49386	pcDNA3 APEX2-NES	Expresses APEX2 in the cytosol of mammalian cells
49385	pcDNA3 Connexin43-GFP-APEX2	Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells