A method for multiprotein assembly in cells reveals independent action of kinesins in complex.
Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, Verhey KJ
J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086. Epub 2014 Nov 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61659 | SNAP-EF(N)-5nmSAH-GFP(1-10) | 5nm SAH scaffold with EF Hand and split GFP attachment sites |
| 61660 | SNAP-EF(N)-10nmSAH-GFP(1-10) | 10nm SAH scaffold with EF Hand and split GFP attachment sites |
| 61661 | SNAP-EF(N)-20nmSAH-GFP(1-10) | 20nm SAH scaffold with EF Hand and split GFP attachment sites |
| 61662 | SNAP-EF(N)-30nmSAH-GFP(1-10) | 30nm SAH scaffold with EF Hand and split GFP attachment sites |
| 61663 | SNAP-EF(N)-60nmSAH-GFP(1-10) | 2x-30nm SAH scaffold with EF Hand and split GFP attachment sites |
| 61664 | KIF5C(1-560)-2xmCherry-EF(C) | Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker |
| 61665 | KIF1A(1-393)-GCN4-2xmCherry-EF(C) | Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker |
| 61667 | KIF5C(1-560)-2xmCitrine-GFP11 | Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker |
| 61670 | KIF5C(1-560)-GFP11 | Non-fluorescent, truncated kinesin-1 fused to split GFP linker |
| 61671 | KIF5C(1-560)-IA | Non-fluorescent, truncated kinesin-1 fused to IA linker |
| 61673 | KIF5C(1-560)-DmrC | Non-fluorescent, truncated kinesin-1 fused to DmrC linker |
| 61675 | KIF5C(1-560)-EF(C) | Non-fluorescent, truncated kinesin-1 fused to EF Hand linker |
| 61676 | KIF5C(1-560)-mCitrine | Single mCitrine-labeled, truncated kinesin-1 |
| 61677 | mEGFP-30nmSAH-GFP(1-10) | GFP-labeled SAH scaffold with split GFP attachment site |
| 61678 | mEGFP-30nmSAH-IQ | GFP-labeled SAH scaffold with IQ attachment site |
| 61679 | mEGFP-EF(N)-30nmSAH | GFP-labeled SAH scaffold with EF Hand attachment site |
| 61680 | mEGFP-30nmSAH-DmrA | GFP-labeled SAH scaffold with DmrA attachment site |