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Compartmentalized AMPK signaling illuminated by genetically encoded molecular sensors and actuators.

Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, Chen M, Tokumitsu H, Zhang J, Inoue T
Cell Rep. 2015 Apr 28;11(4):657-70. doi: 10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61506PM-ABKARExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence
61507Golgi-ABKARExpression of AMPK biosensor targeted to the Golgi
61508ER-ABKARExpression of AMPK biosensor targeted to the endoplasmic reticulum
61509Mito-ABKARExpression of AMPK biosensor targeted to the mitochondria
61510Cyto-ABKARExpression of AMPK biosensor
61511Nuc-ABKARExpression of nuclear AMPK biosensor
61512Tom20-mChF-AIPExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP
61513Tom20-mChF-AIP(TA)Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation
61521Tom20-mChF-AIP(TD)Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutation
61522Tom20-mChF-AIP(TE)Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation
61523AIP(WT)-mChF-giantinExpression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP
61524LAMP-mChF-AIPExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP
61525YF-AIPExpression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIP
61526CF-AIPExpression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIP
61527mChF-AIPExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP
61528mChF-AIP(TA)Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)
61529mChF-AIP (TD)Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)
61530mChF-AIP (TE)Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)
65068LAMP1-ABKARExpression of ABKAR biosensor targeted to the lysozome

Antibodies from Article