The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.
Schiml S, Fauser F, Puchta H
Plant J. 2014 Oct 18. doi: 10.1111/tpj.12704.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61479 | pEn-C1.1 | Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC |