Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
Fauser F, Schiml S, Puchta H
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61432 | pEn-Chimera | Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone |
| 61433 | pDe-CAS9 | Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence |
| 61476 | pChimera | Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone |
| 61477 | pDe-CAS9-D10A | based on pDe-CAS9, encodes Cas9 D10A nickase variant |
| 61478 | pCAS9-TPC | Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning |