Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.

Fauser F, Schiml S, Puchta H
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

Showing 1 to 5 of 5 entries

Show entries

Search table:

ID	Plasmid	Purpose
61478	pCAS9-TPC	Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning
61477	pDe-CAS9-D10A	based on pDe-CAS9, encodes Cas9 D10A nickase variant
61476	pChimera	Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone
61433	pDe-CAS9	Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence
61432	pEn-Chimera	Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone