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Rationally improving LOV domain-based photoswitches.

Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR
Nat Methods. 2010 Aug;7(8):623-6. doi: 10.1038/nmeth.1473. Epub 2010 Jun 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61431LovTAP N538EExpresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.

Antibodies from Article