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Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase.

Matsumura I, Wallingford JB, Surana NK, Vize PD, Ellington AD
Nat Biotechnol. 1999 Jul;17(7):696-701. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
611566his-WT gusA-pET28a+N -terminal His tagged wild-type GUS expression vector
611576his R.3-AR GUSpET28a+/pLysSN-terminal His tagged round 3 aldehyde-resistant GUS expression vector

Antibodies from Article