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Optimization of Critical Hairpin Features Allows miRNA-based Gene Knockdown Upon Single-copy Transduction.

Myburgh R, Cherpin O, Schlaepfer E, Rehrauer H, Speck RF, Krause KH, Salmon P
Mol Ther Nucleic Acids. 2014 Oct 28;3:e207. doi: 10.1038/mtna.2014.58. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61084pENTRdeltaGFPmirGE-DroshaT1.5.5pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Antibodies from Article