Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing.

Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter NG
Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704. Epub 2013 Nov 17. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
61042	Prp2-pRSETA	Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
61043	Cwc25-pRSETA	Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.