MIR144 and MIR451 regulate human erythropoiesis via RAB14.
Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI
Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60934 | miR-144 Trap | to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter |
| 61487 | miR-451 Trap | to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter |
| 61488 | miR-144-451 Trap | to knockdown endogenous miR144 and miR451 levels |
| 61489 | RAB14 3'UTR WT | luciferase reporter containing RAB14 3' UTR |
| 61490 | RAB14 3'UTR D1 | luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted |
| 61491 | RAB14 3'UTR D2 | luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted |
| 61492 | RAB14 3'UTR D1D2 | luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted |
| 61493 | RAB14 WT | to overexpress wild type RAB14. Vector includes GFP driven by hUbC promoter |
| 61494 | RAB14 Q70L | to overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoter |
| 61495 | RAB14 S25N | to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter |
| 61496 | RAB14 shRNA | to knockdown endogenous RAB14 expression levels |