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Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.

Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
60741pZS1[RbsR-L]Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.
60742pZS1[FruR-L]Contains PIq driving expression of the FruR-L, the Fructose inducible chimera with the wt Lac DBD.
60743pzs1[GalS-L]Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.
60745pZS1[LacI-L]Contains PI driving expression dimeric LacI, the Lactose inducible repressor.
60751pZS1[RbsR-T]Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.
60752pZS1[FruR-T]Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.
60753pZS1[GalS-T]Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.
60754pZS1[TreR-T]Contains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.
60756pZS1[Iq:LacI-T]Contains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.
60757pZS1[RbsR-L,LacI-L]Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.
60759pZS1[GalS-L,LacI-L]Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.
60760pZS1[TreR-L,LacI-L]Contains PI driving expression TreR-L, and PI driving expression of LacI-L
60761pZS1[FruR-L,RbsR-L]Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L
60763pZS1[GalS-L,RbsR-L]Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.
60764pZS1[GalS-L,FruR-L]Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.
60765pZS1[GalS-L,TreR-L]Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.
60766pZS1[RbsR-L,TreR-L]Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.
60767pZS1[RbsR-T,LacI-T]Contains PIq driving expression RbsR-T, and PI driving expression of TreR-L.
60768pZS1[FruR-T,LacI-T]Contains PIq driving expression FruR-T, and PI driving expression of LacI-T.
60770pZS1[TreR-T,LacI-T]Contains PI driving expression TreR-T, and PI driving expression of LacI-T.
60772pZS1[FruR-T,TreR-T]Contains PIq driving expression FruR-T, and PIq driving expression of TreR-T.
60773pZS1[GalS-T,RbsR-T]Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.
60775pZS1[GalS-T,TreR-T]Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.
60776pZS1[RbsR-T,TreR-T]Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.
60777pZM3[I:FruR-L,I:RbsR-L]Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.
60778pZA2[PLac:GFP]Contains Plac driving expression of GFP
60779pZA2[PTAN:GFP]Contains PTAN driving expression of GFP
60780pZA2[PLac:mCherry,PTAN:GFP]Contains PLac driving expression of mCherry and PTAN driving expression of GFP.

Antibodies from Article