Selection of chromosomal DNA libraries using a multiplex CRISPR system.
Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, DeLoache W, Dueber JE, Arkin AP, Cate JH
Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
60847 | pCAS | Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast |