CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.
Park A, Won ST, Pentecost M, Bartkowski W, Lee B
PLoS One. 2014 Apr 17;9(4):e95101. doi: 10.1371/journal.pone.0095101. eCollection 2014.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60747 | PuroR-P2A-DD-TCOF1-donor | Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1 |
| 60748 | BlastR-P2A-DD-TCOF1-donor | Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1 |