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Transient sampling of aggregation-prone conformations causes pathogenic instability of a parkinsonian mutant of DJ-1 at physiological temperature.

Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, Cerny RL, Powers R, Wilson MA
Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 2015 Aug 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
60685M26I DJ-1 TEV site pET15bRecombinant protein expression of Park7
60686M26L DJ-1 TEV site pET15bRecombinant protein expression of Park7
60687DJ-1 TEV site pET15bRecombinant protein expression of Park7
60688M26V DJ-1 pET15bRecombinant protein expression of Park7

Antibodies from Article