A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.
Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
BMC Biotechnol. 2014 Sep 25;14(1):84.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59382 | pT2SC | Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene. |
| 59383 | pT2SK | Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene. |
| 59384 | pT2ST | Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene. |
| 59385 | pT2SCb | Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene. |
| 59386 | pSLTS | Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter. |