Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae.

Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
ACS Synth Biol. 2014 Sep 19. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
60619	pCR	crRNA expression plasmid on pRS423 backbone
60620	pCT	Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
60621	pCRCT	Plasmid encoding iCas9, tracrRNA and crRNAs