Effect of fixation procedures on the fluorescence lifetimes of Aequorea victoria derived fluorescent proteins.
Joosen L, Hink MA, Gadella TW Jr, Goedhart J
J Microsc. 2014 Sep 1. doi: 10.1111/jmi.12168.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60491 | pH2B-SCFP1-2A-mTq2-NES | Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm |
| 60492 | 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES | Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm |