A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging.
Chen Y, Saulnier JL, Yellen G, Sabatini BL
Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. eCollection 2014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60445 | AAV-FLEX-FLIM-AKAR | Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone |
| 60446 | AAV-FLEX-FLIM-AKART391A | Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone |
| 63058 | AAV-FLIM-AKAR | Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone |
| 63059 | AAV-FLEX-PKIalpha-IRES-nls-mRuby2 | Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone |
| 63060 | AAV-FLEX-tmeGFP-IRES-nls-mRuby2 | Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone |