Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.
Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60432 | pPB-hCMV1-hNANOG-IV | tet-inducible human NANOG |
| 60433 | pPB-hCMV1-hKLF2-IV | tet-inducible human KLF4 |
| 60434 | pPB-PGK-hESRRB | Over-expression of human ESRRB |
| 60435 | pPB-PGK-hKLF4 | Over-expression of human KLF4 |
| 60436 | pPB-PGK-destination | Destination vector with PGK - Hygromycin selection marker |
| 60439 | pPB-EOS-GFP-IP | PiggyBac for EOS-GFP |
| 60440 | PpyCAG-NANOG-IB | Transient human NANOG expression |
| 60441 | PpyCAG-KLF2-IB | Transient human KLF2 expression |
| 60612 | pPB-CAG-rtTA-IN | Backbone for tet-inducible genes |