Regulation of microtubule motors by tubulin isotypes and post-translational modifications.
Sirajuddin M, Rice LM, Vale RD
Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 2014 Mar 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60385 | pRS426-TUB1 | Expression of yeast alpha tubulin (TUB1) using a GAL promoter |
| 60386 | pRS424-TUB2-Cterminal6xHis | Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter |
| 60387 | pRS424-TUB1-Cterminal6xHis | Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter |
| 60388 | pRS424-TUB2 | Expression of yeast beta tubulin (TUB2) using a GAL promoter |
| 60390 | pRS426-TUB1-internal6xHis | Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter |
| 60391 | pRS426-TUB1-internal6xHis (1-440) | Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter |
| 60392 | pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail | Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter |
| 60393 | pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y | Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y) |
| 60394 | pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C | Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide |
| 60395 | pRS424-TUB2 (1-428) | Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter |
| 60396 | pRS424-TUB2 - human TUBB2a Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter |
| 60397 | pRS424-TUB2 - human TUBB3 Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter |
| 60398 | pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C | Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptide |
| 60399 | pRS424-TUB2 - human TUBB7 Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoter |
| 60400 | pRS424-TUB2 - human TUBB1 Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter |
| 60401 | pRS424-TUB2 - human TUBB8 Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoter |
| 60402 | pRS424-TUB2 - human TUBB4a Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoter |
| 60403 | pRS424-TUB2 - human TUBB6 Cterminal tail | Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter |
| 60404 | pRS424-TUB2 - human TUBB3 Cterminal tail delta K | Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK |
| 60405 | pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C | Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide |
| 60406 | pHR KIF17 (1-738) | Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738 |