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Addgene

From genes to protein mechanics on a chip.

Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash MA, Gaub HE
Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58708pET28a-ybbR-HIS-sfGFP-DocICharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
58709pET28a-ybbR-HIS-CBM-CohIProbing molecule to perform single molecule force spectroscopy experiments
58710pET28a-StrepII-TagRFP-CohIDetection construct to label dockerin type I containing fusion proteins
58711pET28a-ybbR-HIS-Xyl-DocICharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
58712pET28a-ybbR-HIS-10FNIII(x4)-DocICharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
58713pET28a-ybbR-HIS-Spec(x2)-DocICharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Antibodies from Article