MicroRNA-based single-gene circuits buffer protein synthesis rates against perturbations.

Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. Epub 2014 Jan 30. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
59877	sgFFL	synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.
59891	sgFFL_d3	synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.
59892	sgFFL_d23	synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
59893	sgFFL_3x_siRNA	synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites
59894	sgFFL_7x_Bulge	synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
59895	OpenLoopControl	synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR