Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.

Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
Nucleic Acids Res. 2014 Aug 13. pii: gku749. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
84034	pLV GG hUbC-dsRED	Contains Golden Gate cassette to express up to four gRNAs and dsRED
67620	pLV hUbC-dCas9 KRAB-T2A-GFP	Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP
59791	pLV hUbC-VP64 dCas9 VP64-T2A-GFP	Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP
53192	pLV hUbC-dCas9 VP64-T2A-GFP	Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP
53191	pLV hUbC-dCas9-T2A-GFP	Co-expresses human optimized S. pyogenes dCas9 and GFP
53190	pLV hUbC-Cas9-T2A-GFP	3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP
53189	ph7SK-gRNA	Expresses the S. pyogenes sgRNA from the 7SK promoter
53188	phU6-gRNA	Expresses the S. pyogenes sgRNA from the human U6 promoter
53187	pmU6-gRNA	Expresses the S. pyogenes sgRNA from the mouse U6 promoter
53186	phH1-gRNA	Expresses the S. pyogenes sgRNA from the H1 promoter