Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes.
Inoue A, Zhang Y
Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. Epub 2014 Jun 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59745 | pcDNA-ELYS-emerin-EGFP-polyA | Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59746 | pcDNA-EGFP-ELYS-polyA | Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59747 | pcDNA-emerin-EGFP-polyA | Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59748 | pcDNA-LBR-EGFP-polyA | Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only. |
| 59749 | pcDNA-LBR-mRFP1-polyA | Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only. |
| 59750 | pcDNA-RanWT-mRFP1-polyA | Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59762 | pcDNA-Flag-RCC1-polyA | Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59780 | pcDNA-Flag-H3.3-polyA | Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59781 | pcDNA-HIRA-polyA | Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 59782 | pcDNA-HIRAmut-polyA | This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB). |
| 104560 | pcDNA-Ran-Q69L-mRFP1-polyA | Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 104561 | pcDNA-Ran-T24N-mRFP1-polyA | Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |
| 104562 | pcDNA-Nup37-EGFP-polyA | Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. |