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SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme.

Schoene C, Fierer JO, Bennett SP, Howarth M
Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
52656SpyTag-β-Lactamase-SpyCatcherExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

Antibodies from Article