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The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1.

Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
J Biol Chem. 2011 Dec 16;286(50):43352-60. doi: 10.1074/jbc.M111.304089. Epub 2011 Oct 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
59731pET14b_tev_PSGExpresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria
59738pET14b_tev_PSGUExpresses a.a. 417-887 of human ZO-1

Antibodies from Article