High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage.
Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S
Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
51625 | pREDIA | E. coli genome modification |
51626 | pREDKI | E coli Genetic Modification |
51627 | pREDTAI | E coli Genetic Modification |
51628 | pREDTKI | E coli Genetic Modification |
51652 | pMDICI | E coli genetic modification |
51653 | pMDISI | E coli genetic modification |
51655 | pMDIAI | E coli genetic modification |
51725 | pKSI-1 | cloning backbone |