Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF
Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58970 | T7-VEE-IRES-Puro | noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon |
| 58971 | SP6-VEE-IRES-Puro | noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon |
| 58972 | T7-VEE-OKS-iM | RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF |
| 58973 | SP6-VEE-OKS-iM | RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF |
| 58974 | T7-VEE-OKS-iG | RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF |
| 58975 | SP6-VEE-OKS-iG | RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF |
| 58976 | SP6-VEE-GFP | expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon |
| 58977 | T7-VEE-GFP | expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon |
| 58978 | pTNT-B18R | expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs |
| 58979 | pTNT-B18R-6His | expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs |