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Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.

Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58766pX330A-1x2Expresses Cas9 nuclease and gRNA
58767pX330A-1x3Expresses Cas9 nuclease and gRNA
58768pX330A-1x4Expresses Cas9 nuclease and gRNA
58769pX330A-1x5Expresses Cas9 nuclease and gRNA
58770pX330A-1x6Expresses Cas9 nuclease and gRNA
58771pX330A-1x7Expresses Cas9 nuclease and gRNA
58772pX330A_D10A-1x2Expresses Cas9 nickase and gRNA
58773pX330A_D10A-1x3Expresses Cas9 nickase and gRNA
58774pX330A_D10A-1x4Expresses Cas9 nickase and gRNA
58775pX330A_D10A-1x5Expresses Cas9 nickase and gRNA
58776pX330A_D10A-1x6Expresses Cas9 nickase and gRNA
58777pX330A_D10A-1x7Expresses Cas9 nickase and gRNA
58778pX330S-2Expresses Cas9 nuclease and gRNA
58779pX330S-3Expresses Cas9 nuclease and gRNA
58780pX330S-4Expresses Cas9 nuclease and gRNA
58781pX330S-5Expresses Cas9 nuclease and gRNA
58782pX330S-6Expresses Cas9 nuclease and gRNA
58783pX330S-7Expresses Cas9 nuclease and gRNA

Antibodies from Article