Production in Escherichia coli, folding, purification and characterization of notexin with wild type sequence and with N-terminal and catalytic site mutations.
Simonato M, Morbiato L, Zorzi V, Caccin P, Fernandez J, Massimino ML, Polverino de Laureto P, Tonello F
Toxicon. 2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 10.1016/j.toxicon.2014.06.009.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58714 | pET-20b-T7-Ntx | Expresses T7-Notexin in Escherichia coli |
| 58715 | pET-20b-T7-Ntx-K49 | Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K |
| 58716 | pET-20b-M-Ntx-S1 | Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S |