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Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination.

Chesarino NM, McMichael TM, Hach JC, Yount JS
J Biol Chem. 2014 Apr 25;289(17):11986-92. doi: 10.1074/jbc.M114.557694. Epub 2014 Mar 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58458pCMV-myc-mIFITM3Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.
58459pCMV-myc-mIFITM3-deltaY20Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.
58460pCMV-myc-mIFITM3-Y20AExpresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.
58461pCMV-myc-hIFITM3Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.
58462pCMV-myc-hIFITM3-Y20AExpresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.
58463pCMV-myc-hIFITM3-deltaY20Expresses human IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.
58464pCMV-myc-mIFITM3-Y27AExpresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.
58465pCMV-myc-mIFITM3-Y20,27AExpresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.
58537pCAGGS-CD4-mycExpresses human CD4 with a C-terminal myc tag in mammalian cells
58538pCAGGS-CD4-YEML-mycExpresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

Antibodies from Article