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Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.

Mishra P, Carelli V, Manfredi G, Chan DC
Cell Metab. 2014 Apr 1;19(4):630-41. doi: 10.1016/j.cmet.2014.03.011. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58424pclbw-eGFPomp25Mammalian expression of GFP targeted to the mitochondrial outer membrane
58425pclbw-mitoTagRFPMammalian expression of tagRFP targeted to the mitochondrial matrix
58426pclbw-mitoCFPMammalian expression of CFP targeted to the mitochondrial matrix
58427pclbw-PAGFPomp25Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane
62845pclbw-opa1(isoform 1)-mycMammalian Expression of myc-tagged Opa1 isoform1
62846pclbw-opa1(isoform 7DeltaS1)-mycMammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

Antibodies from Article