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Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA.

Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58215pCMV G-CEPIA1erGreen fluorescent indicator for calcium imaging in the endoplasmic reticulum
58216pCMV R-CEPIA1erRed fluorescent indicator for calcium signaling in the endoplasmic reticulum
58217pCIS GEM-CEPIA1erRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum
58218pCMV CEPIA2mtGreen fluorescent indicator for calcium imaging in the mitochondria
58219pCMV CEPIA3mtGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)
58220pCMV CEPIA4mtGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Antibodies from Article