Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint.
Ballister ER, Riegman M, Lampson MA
J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58280 | pERB109 | constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB |
| 58281 | pERB111 | constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB |
| 58282 | pERB122 | const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB |
| 58283 | pERB127 | const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI) |
| 58284 | pERB130 | const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1 |
| 58285 | pERB160 | const. Mis12-FKBP3; ind. FRB-mCH-Mad1 |
| 58286 | pERB161 | const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID) |
| 58288 | pERB170 | const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-584 |
| 58289 | pERB171 | const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718 |
| 58290 | pERB172 | const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA |
| 58291 | pERB174 | const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718 |