Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells.
Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58252 | pAdSh.U6.gRNAS1 | U6 promoter-driven single guide RNA complementary to the human AAVS1 locus |
| 58253 | pAdSh.PGK.Cas9 | Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter |
| 58255 | pAdSh.U6.gRNAGFP | U6 promoter-driven single guide RNA complementary to eGFP |