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Reversible protein inactivation by optogenetic trapping in cells.

Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58366pCMV-CIB1-mCerulean-MPFor use in light-induced protein inactivation through the interaction with CRY2
58367pCMV-CIB1-mRFP1-MPFor use in light-induced protein inactivation through the interaction with CRY2
58368pCMV-CRY2-mCherryMamlian codon optimized CRY2
58369pCMV-CRY2(D387A)-mCherryLight insensitive CRY2 mutant
58370pCMV-SNAP-CRY2-VHH(GFP)For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

Antibodies from Article