Reversible protein inactivation by optogenetic trapping in cells.
Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
58366 | pCMV-CIB1-mCerulean-MP | For use in light-induced protein inactivation through the interaction with CRY2 |
58367 | pCMV-CIB1-mRFP1-MP | For use in light-induced protein inactivation through the interaction with CRY2 |
58368 | pCMV-CRY2-mCherry | Mamlian codon optimized CRY2 |
58369 | pCMV-CRY2(D387A)-mCherry | Light insensitive CRY2 mutant |
58370 | pCMV-SNAP-CRY2-VHH(GFP) | For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP |