A highly effective dominant negative alpha s construct containing mutations that affect distinct functions inhibits multiple Gs-coupled receptor signaling pathways.
Berlot CH
J Biol Chem. 2002 Jun 7;277(23):21080-5. Epub 2002 Apr 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 55797 | G protein alpha-s(alpha3beta5/G226A/A366S) | This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release. |
| 55798 | G protein alpha-s(alpha3beta5/G226A) | In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II. |
| 55799 | G protein alpha-s(alpha3beta5) | This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase. |